ABSTRACT
Objective The objective of this study was to investigate the relationship between serum long-chain non-cod-ing RNA MALAT1 and AFAP1-AS1 and clinicopathological features,and prognosis of nasopharyngeal carcinoma(NPC). Methods A total of 136 patients with nasopharyngeal carcinoma confirmed by pathology in our hospital were selected from April 2013 to June 2015. During the same time,54 outpatients for health examination in our hospital were selected as the control group and nasopharynge-al carcinoma group. Real-time fluorescence reverse transcription analysis was used to analyze the expression of lncRNA MALAT1 and AFP1-AS1. The relationship between the expression of lncRNA MALAT1 and AFP1-AS1,and clinicopathological characteris-tics was analyzed by χ2 test. Log-rank assay was used to analyze serum long-chain non-coding RNA MALAT1 and prognostic differences in patients with different expression levels of AFAP1-AS1. Results Compared with the control group,the serum levels of RNA MALAT1 and RNA AFAP1-AS1 in the nasopharyngeal carcinoma group were significantly increased(P<0. 001);The expres-sions of lncRNA MALAT1 and AFAP1-AS1 was not related to age(P>0. 05);The maximum diameter of the tumor was≥5 cm,the pathological stage was higher,the TNM stage was higher,the deeper in the infiltration depth,the infiltration of lymphatic vessels,the lymph node metastasis,and the recurrence and the higher in high expressive rates of lncRNA MALAT1 and AFAP1 -AS1 ( P <0. 05). The 2-year survival rate and survival time of lncRNA MALAT1 and AFAP1-AS1 in the low expression group were signifi-cantly higher than those of the high expression group(P<0. 001);Multivariate Cox stepwise regression analysis showed that low ex-pression of lncRNA MALAT1(HR=0. 52,95% CI:0. 37~0. 81)and low expression of lncRNA AFAP1-AS1(HR=0. 56,95% CI:0. 51~0. 83)were independent prognostic protective factors for NPC patients(P<0. 001). Conclusion The serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 are elevated in patients with NPC,and are positively correlated with malignant progression of NPC. NPC patients with low expression of lncRNA MALAT1 and AFAP1-AS1 serum have a good prognosis;MALAT1 and AFAP1 -AS1 can be used as new markers for the diagnosis of nasopharyngeal carcinoma.
ABSTRACT
Objective To improve the external irradiation technique for Head-neck neoplasms.Methods 95 patients with Head-neck neoplasms confirmed by pathology,were fixed with head pillow mask , isocenter irradiation,technique utilizing cerrobend block.Radical therapy dose was 72 (70～80)Gy/35～40 fractions in 7～8 weeks for general carcinoma and 50～55Gy/28～31 fractions in 5 6～6 2 weeks for NHL .Preventive dose was 56 (50～60)Gy/25～30 fractions in 5～6 weeks for general carcinoma and 45～50Gy/25～28 fractions in 5～5 6 weeks for NHL.A short and a long-term clinical outcome, acute and late radiation reactions were observed.Results After 1～2 months following radiation therapy,CT scans revealed that the primary lesion complete remission rate was 58 57% , and obvious remission rate was 32 86%.The normal mucous membrane acute radiation reaction rates were G0 56 84% , GⅠ 42 10% , GⅡ 1 05%,respectively.After 1～2 years following radiation therapy,the spinal cord and brain stem radiation injury rate was zoro.Conclusions The improving external irradiation technique may elevate local control rate better,and reduce radiation reactions of patients with head-neck neoplasms.